sambrook fritsch maniatis molecular cloning pdf

The acetylated, radiolabeled forms of chloram- phenicol are separated from unreacted acetyl coenzyme A by partitioning into ethyl acetate. Brooklyn: A Novel From Everand. No serum should be added to this reagent. In practice, several factors other than the speed of mixing affect the size of the precipitate, including the concentration and size of the DNA high-molecular- weight DNA can be sheared by passage through a fine syringe needle and the exact pH of the buffer some workers make up several batches of HBS buffer over the pH range 6. Below, we present detailed protocols for the most commonly used transfection techniques calcium phosphate coprecipitation and DEAE-dextran transfec- tion and we outline alternative methods transfection using Polybrene [page

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