ImageJ2 is structured as an application container, avoiding the static singleton pattern and hence many of ImageJ 1. This first sample data set that has very good colocalization because the 2 subunits of a dimeric protein are stained with green and red dyes respectively. A key advantage of this method type is that the fluorescent signal from the two channels does not need to overlap to demonstrate spatial correlation, which also makes these methods more compatible with super-resolution imaging. Here, as I just recently re- learned, re-thinking our approaches and opinions is an important piece of the puzzle and finally the basis of science. Bat Cochlea Volume.
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