We propose an artificial culture medium supplemented with an iron salt to replace the blood that is traditionally used; it reported successful growth of the bacterial reference strain and of a strain isolated from the environment, which was evaluated by optical density OD , while purity and identity of the culture were checked by using Gram stain, immunofluorescence and PCR. Endoscopy, 39 , pp. Kodama, M. Nishimura, J. Servicios Personalizados Revista.
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