wound healing assay quantification image j software
This can be achieved by making sure that the microscope is set up for Kohler illumination. In such cases, long-term imaging with a fluorescence microscope leads to photobleaching and phototoxicity. You can also use 4x or 5x objective for a larger field of view, while still maintaining a reasonable resolution. The numbers can be exported to an Excel sheet to plot a graph of migration rate versus time or wound closure percentage versus time. Phase contrast is also available on most of the standard and advanced imaging systems.
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