biotinylated dna pcr testing

The format for such hybridization includes binding target 30 nucleic acid to nitrocellulose or nylon membrane dot blot or Southern blot , prehybridization, hybridization to a radiolabelled probe in GuSCN, washing and detection by autoradiography. Thus, the OD of blank wells is subtracted from the OD of sample wells to give an accurate quantification of bona fide capture hybridization. Determination of avidin or avidin-labeled material and determination of biotin or biotin-labeled material. PLoS One 9, Piepenburg, O.

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