After illumination of the nucleus with nanometer light purple lightning bolt; Figure 1 b , the PA-GFP protein exhibits green fluorescence, which slowly diffuses into the cytoplasm, as illustrated by the lower two cell cartoons c and d in the first column. The advancing age of a chimeric protein fused with the timer moiety can be determined by a continual decrease in the observed ratio of green to red fluorescence. This photoactivatable version of GFP was developed by improving on the photoconversion efficiency of the natural wild-type protein chromophore from a predominately neutral form to a species that is anionic in character. Not Available in Your Country Sorry, this page is not available in your country. Among the potential problems with PS-CFP is that, unlike many of the optical highlighters that require a different wavelength for photoactivation and subsequent imaging of the converted species, PS-CFP uses the same wavelength at different intensities for both processes.
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